BABYBOOM CLONING

highly automated tissue culture process under the guidance of babyboom transcription factor

 

 

AUTOMATION

Almost all improvements to quality comes via simplification of design, manufacturing, layout, processes, and procedures.  It distinguishes between a leader and a follower.

 

James Troch, Chief Executive Officer

AUTOMATED TISSUE CULTURE

Plant tissue culture or micropropagation is a method of propagating disease-free clonal plants.  Plant tissue culture is favoured over conventional means such as by cuttings or seeds as it offers various advantages.  Usually micropropagation can be achieved in three ways: (i) by direct organogenesis via shoot tip or axillary buds, (ii) by indirect organogenesis through callus, and (iii) by somatic embryogenesis, indirectly or directly.

Commercial exploitation of tissue culture technology is limited to only a few species because of high cost of the traditional tissue culture production processes.  Here, labour and media constitute more than half of the cost of a tissue culture operation.

Perpetuum developed a new revolutionary cloning process which substantially reduces costs and timelines by devising a photoautotrophic system, which is a method of micropropagation without sugar in the culture medium, in which the growth or accumulation of carbohydrates of cultures is dependent fully upon photosynthesis and inorganic nutrient uptake.  The process utilizes state-of-the-art disposable bag bioreactors to produce somatic embryos en masse.  With improvement in media and other manipulative techniques, like Babyboom Transcription Factor, we can induce somatic embryogenesis at tremendous rates, resulting in higher yields, and faster timelines. 

A key aspect of our cloning platform, is that it is fully automated by using robotic systems for dividing, separating, moving of planting tissues, shoots, somatic embryos and/or plantlets.  This way, little to no human intervention is needed, drastically reducing our overall production cost by as much as 70 percent.

 

WORKFLOW

STARTING MATERIAL

shoot

apical meristem

Virus-free shoot apical meristems are being

used as starting material

 

UPSCALING & DIFFERENTATION

Culturing differentation phase in

wave-like disposable bag bioreactors

 

 

MULTIPLICATION STAGE

Multiplication of embryonic meristems

in shaking incubator

 

 

EMBRYO SORTING AND HARVESTING

Heart stage embryo's are separated from torpedo

stage embryo's by machine vision

 

 

EMBRYO TRANSFER TO GROW STAGE

Embryo's are dropped in to sterile soil or rockwool cubes containing growth medium

 

DIRECT SOMATIC EMBRYOGENESIS INDUCTION

embryonic

meristems

Placed on photoautotrophic medium with the addition of babyboom transcription factor

 

 

OUR OTHER TECHNOLOGIES

GENOME.INSIGHTS.png

GENOMIC

INSIGHTS

Proteins & Peptides.png

TRANSEXPRESS

PLATFORM

tissue.engineering.png

CELL & TISSUE

DESIGN

 

 

genomic.breeding.png

GENOMIC

BREEDING

 

precision.breeding.png

PRECISION

BREEDING

 

NEED MORE INFORMATION?

We are here for you.

Perpetuum Cropscience BVBA - Frans de Cortlaan 3 bus 1, 2610 Antwerpen - Belgium

Email: info@perpetuum-crop.science - Tel.: +32(0)3 435 61 80

 

  • Facebook
  • Twitter
  • Instagram

Copyright © All rights reserved.   Privacy policy   Disclaimer