an in-depth view at nature's treasures
There is no substitute for learning and innovation. They go hand in hand. The arrogance of success is to think that what you did yesterday, will be sufficient for tomorrow. I wake up every morning and think to myself, "how far can i push the company forward in the next 24 hours?"
James Troch, Chief Executive Officer
GENOME INSIGHTS WITH PERPETUUM
Plant genomics is an increasingly important area of science that has expanded in recent years due to the development of advanced technologies and methods. An genuine understanding of plant genomics is a prerequisite for advanced plant breeding and crop improvement. An in-depth knowledge of plant genomics helps researchers to enhance production, confer resistance or tolerance to adverse conditions and improve crops. The recent advances in plant genomics and bioinformatics has had a significant impact on plant science and genetics. New methods and technology have led to a greater understanding of both structural genomics and functional genomics. Plant genomics generates opportunities to create crops with improved traits.
Our expertise areas are:
GENOME SEQUENCING AND ASSEMBLY
Perpetuum's expertise in the latest sequencing technologies and bioinformatics solutions, combined with long-read sequencing on the Illumina platform, enable us to deliver high-quality data and analysis for the genomes of plants, bacteria, and viruses.
DE NOVO ASSEMBLY
Assemble novel genomes without a reference sequence
Discover >50 bp INDELs, translocations, inversions, and copy number variations
Identify SNVs, short INDELs, and germline/somatic mutations
Determine which alleles are co-located on the same chromosome
ULTRA HIGH MOLECULAR WEIGHT DNA ISOLATION
One of the most critical components of successful long read sequencing is working with high purity, ultra-high molecular weight (uHMW) DNA. Preparing this material is particularly challenging in plants, due to the cell wall and the presence of diverse factors such as secondary metabolites, pigments, polysaccharides, etc. This means that there is no generic protocol that can be used. Working with the highly automated oKtopure™ DNA extraction platform, Perpetuum has considerable experience and expertise in isolating uHMW DNA from a range of different plant species, resulting in high quality sequencing results.
The oKtopure™ is a robot platform which combines standardised high-throughput DNA extraction from plant tissue with its proprietary sbeadex™ magnetic bead based extraction chemistry. Key to the delivery of consistently high quality and high yield DNA preparations from the oKtopure is its ability to bind and hold the magnetic beads, and hence the DNA in place whilst transferring the buffers required for washing, binding and elution steps. The final aqueous wash used in the sbeadex extraction protocols enables the delivery of nucleic preparations ideal for downstream applications such as SNP genotyping and NGS and Sanger sequencing.