CELL & TISSUE DESIGN

optimizing regeneration abilities of

plant cells and tissues

 

CROP IMPROVEMENT & GENE EDITING

Easy DNA editing will remake and improve the world. Buckle up!

 

Jeroen Hofenk, Chief Science Officer

CELL & TISSUE DESIGN IN PLANTS

Within our cell & tissue design platform, Perpetuum develops methodologies to optimize the regeneration abilities of plant cells and tissues. This enables precision breeding and delivers novel biological concepts for seed production and variety/cultivar development. 

Perpetuum provides key solutions for cell biology bottlenecks associated with creation of genome edited crops in plants which are difficult to regenerate from single cells to a fertile stage, thereby creating elite crops or strains with improved complex traits, such as reproduction traits, biotic and abiotic stress, via creation of novel plant tissues.

 

Perpetuum has state-of-the-art protoplast and cytoplast protocols for plant regeneration and genome editing.

PROTOPLAST FUSION APPROACH

Protoplasts are plant cells which have lost their cell wall. They are generated by treating parts of leaves with enzymes which digest the cell wall. The protoplasts form new cell walls and divide, forming a callus and later a plant. Chemical or electrical stimuli can be used to stimulate protoplasts from different plant species to fuse (somatic hybridization).

 

In the course of the fusion the organelles of both plants (chloroplasts and mitochondria) combine while during crossing the descendants only receive the maternal chloroplasts and mitochondria. The tetraploid plant resulting from this fusion shows the characteristics of both parental plants. During regeneration, the chromosomes and organelles of parents can become mixed, resulting in many new combinations.

PLANT SOURCE ONE

Cut leaf as starting material

 

PLANT SOURCE TWO

Cut leaf as starting material

 

ENZYME TREATMENT TO

DISSOLVE CELL WALLS

PROTOPLASTS

CHEMICAL OR ELECTRICAL

STIMULI

CELL FUSION

 

CELL NUCLEUS FUSION

 

CYTOPLAST FUSION APPROACH

In order to avoid the exchange of chromosomes the protoplasts can be treated in such a way that the nucleus is removed or fragmented. These so-called cytoplasts contain the organelles but no chromosomes from the donor plant (parent plant).  In this way one can, for example, transfer CMS (cytoplasmic male sterility) into another plant. 

CELL WITHOUT NUCLEUS

(CYTOPLASTS)

PROTOPLASTS

 

CHEMICAL OR ELECTRICAL

STIMULI

CELL FUSION

 

NEW CELLS

 

OUR OTHER TECHNOLOGIES

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GENOMIC

INSIGHTS

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TRANSEXPRESS

PLATFORM

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BABYBOOM

CLONING

 

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GENOMIC

BREEDING

 

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PRECISION

BREEDING

 

NEED MORE INFORMATION?

We are here for you.

Perpetuum Cropscience BVBA - Frans de Cortlaan 3 bus 1, 2610 Antwerpen - Belgium

Email: info@perpetuum-crop.science - Tel.: +32(0)3 435 61 80

 

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